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KMID : 0604520220480040321
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Journal of the Society of Cosmetic Scientists of Korea
2022 Volume.48 No. 4 p.321 ~ p.330
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Analytical Method of Multi-Preservatives in Cosmetics using High Performance Liquid Chromatography
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Lee Min-Jeong
Kim Seong-Soo
Lee Yun-Jeong
Lee Byeong-Chul
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Abstract
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This study attempted to establish an optimal multi-compound simultaneous analysis method that can secure reliable results for 15 - preservatives, 2 - sun screens and 1 - antioxidants of cosmetics using HPLC-PDA. Since the potential of hydrogen (pH) in the mobile phase affects the acid dissociation constant (pKa) of the preservatives, and the peak retention time shift and area change were observed. The peak separation condition was established by adjusting the pH to 0.1% H3PO4 addition (mL) when preparing the mobile phase. As a results of method validation, the linearity correlation coefficient (R2 ) of above 0.999 were obtained, and accuracy 87.9 ～ 101.1%, 0.1 ～ 7.6% precision for two types of cosmetics (cream and shampoo). It was found that the limit of detection (LOD) was 0.1 ～ 0.2 mg/kg and the limit of quantitation (LOQ) was 2.0 ～ 4.0 mg/kg. In addition, it was possible to simultaneously separate p-anisic acid, a natural compound that was difficult to separate in HPLC due to the small difference from methylparaben, a synthetic preservatives. Through this study, it will be effectively used to secure quality control and safety for compound that need restrictions on use cosmetics.
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KEYWORD
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preservatives, pKa, pH, mobile phase, HPLC
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